CB1626

Jurkat FcγRIIIa (CD16a) F176 NFAT-Luc

£2,000.00
£2,000.00
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A Jurkat cell line expressing the FcγRIIIa (CD16a) F176 receptor and an NFAT-luciferase reporter, used to assess CD16 engagement (ADCC).

FOR RESEARCH USE ONLY

RoukenCells product packaging on a light pink background
Graph comparing Jurkat WT and Jurkat FcγRIIIa (CD16a) F176 NFAT cell populations on a CD16a axis.
  • RoukenCells product packaging on a light pink background
  • Graph comparing Jurkat WT and Jurkat FcγRIIIa (CD16a) F176 NFAT cell populations on a CD16a axis.
RoukenCells product packaging on a light pink background
Graph comparing Jurkat WT and Jurkat FcγRIIIa (CD16a) F176 NFAT cell populations on a CD16a axis.
Specification
Target/Receptor
CD16a (F176)
Aliases
FcγRIIIa, Low affinity immunoglobulin gamma Fc region receptor III-A
Uniprot ID
P08637
Receptor Species
Homo sapiens
Response Element
NFAT
Reporter
Firefly Luciferase
Cell Line Background
Jurkat
Target Information and Further Details
This Jurkat cell line stably expresses human FcγRIIIa (CD16a) F176 (low-affinity, polymorphic variant of CD16a) along with a firefly luciferase reporter driven by NFAT response elements upstream of a minimal promoter. Activation of NFAT can be quantitatively assessed through luciferase activity, providing a robust and sensitive readout for pathway stimulation. FcγRIIIa (CD16a) F176 expression has been confirmed by flow cytometry, and the cell line has been functionally validated in co-culture assays.
Performance Data
Graph comparing Jurkat WT and Jurkat FcγRIIIa (CD16a) F176 NFAT cell populations on a CD16a axis.

Figure 1. CD16 expression profile (orange) compared to wild-type (grey).

Figure 2. ADCC assay with Rituximab (3000 – 0.01 ng/mL). Jurkat FcγRIIIa (CD16) F176 reporter cells co-cultured with Raji at 5:1 E:T for 24 hrs. EC50 = 6.221

Shipping Details

Supplied as 1 vial of >3 million viable cells.

Specification Sheet

Access detailed product specifications, user guides and technical documentation in PDF format.

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