Our cell products are compatible with in vitro assays
Our continually updated in-house catalogue includes a wide range of pre-developed cell lines including IndEx‑2, our flagship inducible cell line technology, reporter cell lines, and primary immune cell banks.
We have designed these to integrate seamlessly into functional assay workflows, supporting applications such as mechanism‑of‑action studies, potency assays, screening and more.
IndEx-2 Inducible Cell Lines:
Evaluate the Impact of Target Antigen Density on Immunotherapeutics
The IndEx-2 assay system is a robust in vitro model to evaluate the influence of antigen expression levels on a targeted therapeutic’s efficacy and safety. This versatile tool enables the controlled expression of one or two antigens of interest and uniquely allows for the estimation of antigen expression thresholds required for therapeutic activation.
- Model pathological and physiological expression levels, by titrating the expression of one, or two, TAOIs over a dynamic range that encompasses biologically relevant expression levels.
- Determine the impact of antigen density on the efficacy and safety of targeted therapies in the same cell line, negating confounding genetic background differences.
- Select therapeutic candidates with lower chance of antigen escape by determining the activation threshold or minimum number of receptors for therapeutic efficacy.
- Evaluate the risks associated with ‘on-target/off-tumour’ toxicities in a pre-clinical setting.
LocIn:
Targeted Integration for High-Fidelity Cell Line Generation
LocIn is an advanced targeted integration system designed for the efficient generation of cell lines by retargeting pre-defined genomic loci, known as "landing pads." These landing pads incorporate a fluorescent protein marker, enabling the easy identification of integrations at genomic sites that support high transgene expression.
The system leverages Recombinase Mediated Cassette Exchange (RMCE) to precisely replace the landing pad cassette with a gene of interest.
This approach ensures the complete removal of the original cassette during retargeting while exclusively integrating the desired gene. By eliminating positional and context-dependent effects, LocIn enables accurate studies of phenotypic outcomes, delivering reliable genotype-to-phenotype correlations.
Confidence in every vial
Our advanced cell products combine cell engineering expertise with robust QC and assay‑ready design principles. We ensure therapeutic developers can rely on consistent performance and clear decision‑making data throughout drug discovery.
Custom IndEx-2 inducible cell lines
Independently modulate one or two antigens to map activation thresholds across biologically relevant densities. Ideal for de-risking candidate MoA and tuning functional readouts.
Reporter cell lines
Pathway-aligned reporters that capture signalling changes linked to therapeutic mechanisms, enabling assay-ready readouts.
Where RoukenCells products fit in your workflow
Our assay-ready cell products mean your teams can plug engineered cells or immune cell banks directly into high-value discovery phases.
- Mechanism-of-action studies
- Potency assay development/qualification
- High-throughput screening
- Regulatory alignment
