CB1631

Jurkat FcγRIIa (CD32a) H167 NFAT-Luc

£2,000.00
£2,000.00
Tax included.
In Stock

A Jurkat cell line with the FcγRIIa (CD32a) H167 variant and an NFAT-luciferase reporter, used to assess CD32 engagement (ADCP).

FOR RESEARCH USE ONLY

RoukenCells product packaging on a pink background
  • RoukenCells product packaging on a pink background
RoukenCells product packaging on a pink background
Specification
Target/Receptor
CD32a (H167)
Aliases
FcγRIIa, Low affinity immunoglobulin gamma Fc region receptor II-a
Uniprot ID
P12318
Receptor Species
Homo sapiens
Response Element
NFAT
Reporter
Firefly Luciferase
Cell Line Background
Jurkat
Target Information and Further Details
This Jurkat cell line stably expresses human FcγRIIa (CD32a) H167 (high-affinity, polymorphic variant of CD32a) along with a firefly luciferase reporter driven by NFAT response elements upstream of a minimal promoter. Activation of NFAT can be quantitatively assessed through luciferase activity, providing a robust and sensitive readout for pathway stimulation. FcγRIIa (CD32a) H167 expression has been confirmed by flow cytometry, and the cell line has been functionally validated in co-culture assays.
Performance Data

Figure 1. CD32a expression profile (orange) compared to wild-type (grey).

Figure 2. ADCC assay with Rituximab (3000 – 0.05 ng/mL).
Jurkat FcγRIIa (CD32a) H167 reporter cells co-cultured with Raji at 5:1 E:T for 24 hrs.

Shipping Details

Supplied as 1 vial of >3 million viable cells.

Specification Sheet

Access detailed product specifications, user guides and technical documentation in PDF format.

Download PDF

Related products

Take a moment to explore selections tailored just for you.

Explore Now