CB1637

HEK293 STAT6-Luc

£2,000.00
£2,000.00
Tax included.
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A HEK293 cell line expressing a STAT6 response element lucifersae reporter. Engineered to study IL-4/IL-13 receptor signalling through STAT6 activation.

FOR RESEARCH USE ONLY

White tube with text on a light pink background
Graph showing luminescence (RLU) on the y-axis against IL-4 concentration (ng/mL) on the x-axis, with a focus on IL-4.
Graph showing luminescence (RLU) against IL-13 concentration (ng/mL) on a log scale.
Graph showing Luminescence (RLU) against Dupilumab concentration with two IL-4 concentrations.
Graph showing luminescence (RLU) against Dupilumab concentration with IL-13 levels.
  • White tube with text on a light pink background
  • Graph showing luminescence (RLU) on the y-axis against IL-4 concentration (ng/mL) on the x-axis, with a focus on IL-4.
  • Graph showing luminescence (RLU) against IL-13 concentration (ng/mL) on a log scale.
  • Graph showing Luminescence (RLU) against Dupilumab concentration with two IL-4 concentrations.
  • Graph showing luminescence (RLU) against Dupilumab concentration with IL-13 levels.
White tube with text on a light pink background
Graph showing luminescence (RLU) on the y-axis against IL-4 concentration (ng/mL) on the x-axis, with a focus on IL-4.
Graph showing luminescence (RLU) against IL-13 concentration (ng/mL) on a log scale.
Graph showing Luminescence (RLU) against Dupilumab concentration with two IL-4 concentrations.
Graph showing luminescence (RLU) against Dupilumab concentration with IL-13 levels.
Specification
Target/Receptor
None
Aliases
N/A
Uniprot ID
N/A
Receptor Species
N/A
Response Element
STAT6
Reporter
Firefly Luciferase
Cell Line Background
HEK293
Target Information and Further Details
This HEK293 cell line expresses a STAT6 response element driving a luciferase reporter. It can be used to study IL-4/IL-13 receptor signalling through STAT6 activation.
Performance Data
Graph showing luminescence (RLU) on the y-axis against IL-4 concentration (ng/mL) on the x-axis, with a focus on IL-4.

Figure 1. Activation assay with IL-4 and IL-13. HEK293 STAT6-Luc Reporter cells were seeded at 7,500 cells/well and incubated with IL-4 (1.8-fold dilution series; 1 - 0.000025 ng/mL) or IL-13 (3-fold dilution series; 1 - 0.00000565 ng/mL) for 22 hrs.

Graph showing luminescence (RLU) against IL-13 concentration (ng/mL) on a log scale.

Figure 1. Activation assay with IL-4 and IL-13. HEK293 STAT6-Luc Reporter cells were seeded at 7,500 cells/well and incubated with IL-4 (1.8-fold dilution series; 1 - 0.000025 ng/mL) or IL-13 (3-fold dilution series; 1 - 0.00000565 ng/mL) for 22 hrs.

Graph showing Luminescence (RLU) against Dupilumab concentration with two IL-4 concentrations.

Figure 2. IL-4Rα blocking assay with Dupilumab (1000 – 0.00000774 ng/mL). HEK293 STAT6-Luc Reporter cells pre-incubated with Dupilumab for 1 hr prior to incubation with set concentrations of IL-4 or IL-13 for 22 hrs.

Graph showing luminescence (RLU) against Dupilumab concentration with IL-13 levels.

Figure 2. IL-4Rα blocking assay with Dupilumab (1000 – 0.00000774 ng/mL). HEK293 STAT6-Luc Reporter cells pre-incubated with Dupilumab for 1 hr prior to incubation with set concentrations of IL-4 or IL-13 for 22 hrs.

Shipping Details

Supplied as 1 vial of >3 million viable cells.

Specification Sheet

Access detailed product specifications, user guides and technical documentation in PDF format.

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