CB1587

CHO-K1 MIC-A/B

£2,000.00
£2,000.00
Tax included.
Out of Stock

A CHO-K1 MIC-A/B expressing cell line which is used with Jurkat NKG2D NFAT-luciferase reporter.

FOR RESEARCH USE ONLY

Rouken Cells container on a light gray background
Graph comparing CHO-K1 WT and CHO-K1 MIC-A/B cell populations on a white background
Graph showing luminescence (RLU) against E:T ratio for an activation assay with Jurkat NKG2D NFAT-Luc cells.
  • Rouken Cells container on a light gray background
  • Graph comparing CHO-K1 WT and CHO-K1 MIC-A/B cell populations on a white background
  • Graph showing luminescence (RLU) against E:T ratio for an activation assay with Jurkat NKG2D NFAT-Luc cells.
Rouken Cells container on a light gray background
Graph comparing CHO-K1 WT and CHO-K1 MIC-A/B cell populations on a white background
Graph showing luminescence (RLU) against E:T ratio for an activation assay with Jurkat NKG2D NFAT-Luc cells.
Specification
Target/Receptor
MIC-A
MIC-B
Aliases
PERB11.1, MHC class I polypeptide-related sequence A
PERB11.2, MHC class I polypeptide-related sequence B
Uniprot ID
Q29983
Q29980
Receptor Species
Homo sapiens
Homo sapiens
Response Element
None
Reporter
None
Cell Line Background
CHO-K1
Target Information and Further Details
This CHO-K1 cell line stably expresses human MHC class I polypeptide-related sequence A (MIC-A) and human MHC class I polypeptide-related sequence B (MIC-B). When co-cultured with Jurkat NKG2D NFAT-Luc cells (RoukenCells #CB1586), activation of NFAT can be quantitatively assessed through luciferase activity, providing a robust and sensitive readout for pathway stimulation. MIC-A and MIC-B expression have been confirmed by flow cytometry, and the cell line has been functionally validated in co-culture assays.
Performance Data
Graph comparing CHO-K1 WT and CHO-K1 MIC-A/B cell populations on a white background

Figure 1. MIC-A/B expression profile (orange) compared to wild-type (grey)

Graph showing luminescence (RLU) against E:T ratio for an activation assay with Jurkat NKG2D NFAT-Luc cells.

Figure 2. Activation assay with Jurkat NKG2D NFAT-Luc (1:1 – 1:0.002 E:T). Jurkat NKG2D NFAT-Luc reporter cells co-cultured with CHO-K1 MIC-A/B for 24 hrs.

Shipping Details

Supplied as 1 vial of >3 million viable cells.

* Please note that US import permits for CHO-K1 cell products are the responsibility of the US-based recipient/importer. RoukenCells is happy to provide supporting documentation (e.g. product descriptions and noninfectious statements) to assist with the import process.

Cell Line Datasheet

Access detailed product specifications, user guides and technical documentation in PDF format.

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