CB1647

RBL-2H3 hFCεR1α NFAT-Luc

£5,000.00
£5,000.00
Tax included.
In Stock

A FcRepsilon and NFAT-Luciferase expressing rat mast cell line. Combined with IgE sensitisation and crosslinking protocols, it can be used to screen molecules that can impact FcεRI triggering.

FOR RESEARCH USE ONLY

Specification
Target/Receptor
IgE Fc receptor subunit alpha
IgE Fc receptor subunit beta
IgE Fc receptor subunit gamma
Aliases
FCER1A, High affinity immunoglobulin epsilon receptor subunit alpha
MS4A2, FCER1B, High affinity immunoglobulin epsilon receptor subunit beta
FCER1G, High affinity immunoglobulin epsilon receptor subunit gamma
Uniprot ID
P12319
Q01362
P30273
Receptor Species
Homo sapiens
Homo sapiens
Homo sapiens
Response Element
NFAT
Reporter
Firefly Luciferase
Cell Line Background
RBL-2H3
Target Information and Further Details
This RBL-2H3 (rat) mast cell line expresses human high affinity immunoglobulin epsilon receptor subunits alpha, beta and gamma, forming a complete Fc epsilon receptor (FcεR1), and a NFAT response element driving a luciferase reporter. When combined with IgE sensitisation and crosslinking protocols, it can be used to screen molecules that can impact FcεRI triggering. FcεRI expression has been quantified by flow cytometry and the cell line has been functionally validated for signalling in response to IgE/allergen cross-linking.
Performance Data

Figure 1. FcεRIα expression profile (orange) compared to wild-type (grey).

Figure 2. IgE Neutralisation Assay with Omalizumab (50000 – 3.05 ng/mL) and Anti-ara h2 IgE (70 ng/mL). RBL-2H3 hFCεR1α reporter cells sensitised with antibodies overnight prior to crosslinking with LoTox™ Peanut Flour Protein for 3 hours.

Shipping Details

Supplied as 1 vial of >3 million viable cells.

Specification Sheet

Access detailed product specifications, user guides and technical documentation in PDF format.

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